Cat. No.
MABL-2427
Application
IP, NTRL, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Japanese Encephalitis Virus, Murray Valley encephalitis virus
Clone No.
M2-8E7
From
Recombinant Antibody
Specificity
This antibody reacts with domain III of the envelope protein of the Murray Valley encephalitis virus. The mAb also cross reacts with Japanese encephalitis virus.
Alternative Names
Murray Valley encephalitis virus; envelope protein; E protein; Japanese encephalitis virus
UniProt
P05769
Immunogen
The original antibody was generated by immunizing BALB/c mice with structural and non structural viral proteins using a crude suspension of infected suckling mouse brain.
Application Notes
This neutralizing antibody binds the the envelope protein of the Murray Valley encephalitis virues in the ectodomain III region. This mAb is reported to block haemagglutination. The competitive binding of the mAb to envelope protein was checked with ELISA (PMID:1703213). Recombinant MVEV E-DIII proteins were detected by M2-8E7 in a western blot assay (PMID: 29083957). This antibody is also reported to be used in immunoprecipitation for the identification of E proteins from cell lysates (PMID: 9770429, 9499070).
Antibody First Published
Hall et al. Epitope analysis of the envelope and non-structural glycoproteins of Murray Valley encephalitis virus J Gen Virol (1990);71 ( Pt 12):2923-30. PMID:1703213
Note on publication
Describes the spatial arrangement, conformation, viral specificity and biological properties of epitopes recognized by a panel of MAbs produced to the E and NS1 proteins of MVEV.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
