Cat. No.
MABL-2430
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Avian, Human, Mouse
Clone No.
33
From
Recombinant Antibody
Specificity
This antibody recognizes the human proto-oncogene c-myc, a growth factor which is overexpressed in cancer cells.
Alternative Names
Transcription factor p64; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; C-33
UniProt
P01106
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing mice against the full-length human c-myc protein. The spleen cells of these mice were then fused with mouse myeloma.
Application Notes
This original mouse IgG1 can be used to detect the human myc-c growth factor. The specificity of this antibody has been confirmed using immunoprecipitation and western blotting (Zhang et al., 2008; PMID: 18438430). In addition, one group used a western blot assay to research the myc-c levels in cells at different stages of cell growth (Shichiri et al., 1993; PMID: 8494788). Another group used western blotting to visualize the amount of myc-c in different tumours (Marin et al., 1995; PMID: 7698223). A final group used immunohistochemistry to analyze the role myc-c plays in tumour development (Banerjee et al., 1996; PMID: 8631582).
Antibody First Published
Shichiri et al. Effects of c-myc expression on proliferation, quiescence, and the G0 to G1 transition in nontransformed cells Cell Growth Differ. 1993 Feb;4(2):93-104 PMID:8494788
Note on publication
Is the first mention of the original mouse IgG1 antibody and describes its usuage
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.