Cat. No.
MABL-2466
Application
bactericidal, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Escherichia coli, Neisseria meningitidis
Clone No.
HmenB3
From
Recombinant Antibody
Specificity
This antibody binds to the capsular polysaccharide of both Neisseria meningitidis group B and E. coli K1. It also reacts with EV36, a nonpathogenic E. coli K-12 strain
Alternative Names
CPS 6B; capsular PS B
UniProt
Immunogen
This antibody was raised by immunising BALB/cByJ mice with E. coli K1 bacteria (strain RS218). The E. coli K1 capsular polysaccharide antigen shows structural and immunological identity with the meningococcal group B antigen, as both are α(2-8)-linked p
Application Notes
The binding characteristics of this antibody have been assessed in flow cytometric analysis; this antibody binds to N. meningitidis group B and E. coli EV36 (Shin et al, 2001). It does not react with N. meningitidis groups A, C, and Y, and is non-autoreactive, as it does not react with human neuronal α(2-8) polysialic acid (Shin et al, 2001). This antibody has been used in a sandwich type ELISA to investigate the cross-reactivity of bacteriophage clones displaying the antigenic properties of serotype 6B pneumococcal capsular polysaccharide (Shin et al, 2002). In the presence of rabbit and human complement, this antibody exhibits bactericidal activity (Shin et al, 2001).
Antibody First Published
Shin et al. Monoclonal antibodies specific for Neisseria meningitidis group B polysaccharide and their peptide mimotopes. Infect Immun. 2001 May;69(5):3335-42. PMID:11292756
Note on publication
Describes the original generation of this antibody, and its characterisation in ELISA analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
