Cat. No.
MABL-2491
Application
crystallography
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
17
From
Recombinant Antibody
Specificity
Reacts specifically with rat GluN2B ATD.
Alternative Names
NMDAR; NMDA-R; Glutamate receptor
UniProt
Q00960
Immunogen
Monoclonal antibodies that bind rat GluN2B ATD were obtained by immunizing mice with the purified intact GluN1-GluN2B NMDA receptors. IgGs were purified from hybridoma cell culture supernatant by Protein-A Sepharose. Fab fragments have been prepared by papain proteolysis followed by re- chromatographing onto Protein-A Sepharose to remove Fc.
Application Notes
This antibody has been used for the crystallisation of the NMDA receptor in the absence of an allosteric inhibitor at 2.9A (Tajima 2016).
Antibody First Published
Tajima et al. Activation of NMDA receptors and the mechanism of inhibition by ifenprodil Nature. 2016 May 2; 534(7605): 63–68 PMID:27135925
Note on publication
Describes the lack of high resolution structures representing the active state of the NMDA receptor.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
