Cat. No.
MABL-2493
Application
WB, IF
Isotype
Engineer antibody
Species Reactivity
Drosophila, Rat, Human
Clone No.
SF9
From
Recombinant Antibody
Specificity
This antibody is specific for a very small (29 aa long; 3 kDa) epitope of non muscle Myosin IIA, starting at aa 1536 in the full human sequence.
Alternative Names
nmMyoIIA; NMMHC II-a; NMMHC-IIA; NMMHC-A; Non-muscle myosin heavy chain Iia; Myosin-9; Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle Iia; Non-muscle myosin heavy chain A
UniProt
Immunogen
The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver.
Application Notes
The original format of this antibody (scFv) was successfully used in a Western blot and in for in vitro immunofluorescence staining of non muscle Myosin IIA (nmMyoIIA) in HeLa cells (Nizak et al., 2003; PMID: 14617357). Precise characterization of the SF9 target was achieved, defining a very small (3 kDa) epitope within the ~200kDa nmMyoIIA protein, revealing cross-reactivity with closely related proteins of the same and across species (Vielemeyer et al., 2010; PMID: 20727208).
Antibody First Published
Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11):739-53. doi: 10.1034/j.1600- 0854.2003.00132.x PMID:14617357
Note on publication
The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
