Cat. No.
MABL-2499
Application
crystallization, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Norovirus
Clone No.
5B18
From
Recombinant Antibody
Specificity
The antibody binds to a face of the GII.10 P1 subdomain close to the S domain and not openly exposed at the VLP surface (residues Val433, Glu496, Asn530, Tyr533, Thr534, and Leu535). The antibody cross-reacts with three GII genotypes GII.4, GII.10, and GII.12 but not with GI genotypes.
Alternative Names
Capsid protein
UniProt
Q5F4T5
Immunogen
The antibody was generated from a mouse immunized with GII.4 norovirus-445 VLPs.
Application Notes
The specificity of the IgG format of this antibody was confirmed by ELISA analysis; the antibody detected intact GII.10 VLPs at different pH values with nearly identical efficacy. The structure of the Fab fragment in complex with GII.10 P domain was determined. The Western blotting results showed that the antibody IgG could detect the three GII genotypes (Hansman et al., 2012; PMID: 22278249). The antibody was used for immunofluorescence microscopy to examine VLP binding to fresh biopsies of human ileum and it bound specifically to VLPs (Murakami et al., 2013; PMID: 23799113).
Antibody First Published
Hansman et al. Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle. J Virol. 2012 Apr; 86(7): 3635–3646. PMID:22278249
Note on publication
The paper describes the crystal structure and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
