Cat. No.
MABL-2502
Application
ELISA; blocking
Isotype
Engineer antibody
Species Reactivity
Norovirus
Clone No.
12B10
From
Recombinant Antibody
Specificity
The antibody is specific for Norovirus GII.4. It shows intra-genogroup cross-reactivity to genotypes 1, 4, 6, and 7 of genogroup II.
Alternative Names
UniProt
Immunogen
A phage-displayed antibody library was generated from peripheral blood of healthy persons. The original antibody was isolated by screened using purified virus-like particles (VLPs) of strain Narita 104 (r104, genogroup II, genotype 4).
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The blocking activity of the antibody against the interaction of homotypic VLPs with histo-blood group antigens were also assessed as an alternative to neutralization assay. The antibody prevented the binding of r104 to histo-blood group antigens that had been reported to bind r104.
Antibody First Published
Higo-Moriguchi et al. Isolation of cross-reactive human monoclonal antibodies that prevent binding of human noroviruses to histo-blood group antigens J Med Virol. 2014 Apr;86(4):558-67. PMID:24026909
Note on publication
The original paper identified a panel of human monoclonal antibodies targeting norovirus.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
