Cat. No.
MABL-2536
Application
immunoblot, in vitro, inhibition, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
67-2
From
Recombinant Antibody
Specificity
This antibody is specific for the peptide ALCNQFYTPAATGLYVD, which is part of the second extracellular loop (EL2) of human OCLN (aa 214-230). However, it has also been shown to bind mouse OCLN, strongly suggesting that it does not recognize the human-specific amino acids in OCLN-EC2.
Alternative Names
OCLN
UniProt
Q16625
Immunogen
The original antibody was generated by immunizing mice with KLH-coupled, modified hOCLN- EC2 peptide. The modification made to the hOCLN-EC2 peptide was an added cysteine residue was at the N- terminal end.
Application Notes
The original format of this antibody (mouse IgG1) can bind hOCLN, as was determined by ELISA during initial hybridoma screening. This antibody efficiently inhibited HCV infection in the HCV- permissive human cell line Huh7.5.1. This antibody can access OCLN from the basolateral side of hepatocytes but not from the apical side using three different culture systems of Huh7.5.1 cells. This antibody was shown to cross-react with mouse OCLN in a WB. This antibody showed no adverse effects on cell viability or the barrier function of tight junctions (Okai et al., 2018; PMID: 29682171).
Antibody First Published
Okai et al. A novel occludin-targeting monoclonal antibody prevents hepatitis C virus infection in vitro Oncotarget. 2018 Mar 30;9(24):16588-16598. doi: 10.18632/oncotarget.24742. PMID:29682171
Note on publication
The original publication discusses the development of an anti-human occludin (hOCLN) monoclonal antibody (mAb) 67-2 and its potential as a target for preventing and treating HCV infection.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
