Cat. No.
MABL-2559
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Neisseria meningitidis
Clone No.
CB-NM.2
From
Recombinant Antibody
Specificity
This antibody is specific for a five amino acid region (S/T)VETG located in the main variable region (VR) VR1 which was present in all N. meningitidis strains. It recognizes class 3 outer membrane protein of Neisseria meningitidis. This antibody can recognize fifty six strains belonging to seven serogroups: A, B, 29E, L, X, Y, Z, and five serotypes: 1, 4, 5, 12, 13 but did not recognize the serotypes 2, 6, 9, 11, 14, 15, 18 and 19.
Alternative Names
class 3 outer membrane protein; 15F9; NM-2
UniProt
Immunogen
The original antibody was generated by immunizing mice with serotype antigen preparations from case isolate B385 strain of Neisseria meningitidis, from Havana.
Application Notes
The binding reactivity of this antibody against fifty six strains of Neisseria meningitidis belonging to seven serogroups: A, B, 29E, L, X, Y, Z, and five serotypes: 1, 4, 5, 12, 13 was assessed using whole cell ELISA. This antibody can also be used in the detection of these serogroups and serotypes using western blot (Guillen et al., 1997).
Antibody First Published
Guillen et al. Molecular analysis Neisseria meningitidis class 3 outer membrane protein in strains recognized by the monoclonal antibody CB-Nm.2. Biotechnologia Applicada 1997; Vol -14:23-30.
Note on publication
This paper analyzes the reactivity of this antibody with various serogroups and serotypes of N. meningitidis and determines the binding epitope of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
