Cat. No.
MABL-2580
Application
in vivo, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
1E 10
From
Recombinant Antibody
Specificity
This antibody is specific for the antigen binding site of the anti-NeuGcGM3 antibody P3 (Ab04722).
Alternative Names
Mabai 1E10
UniProt
Immunogen
the original antibody was generated by immunizing a BALB/c mouse with KLH-coupled P3 Mab.
Application Notes
The original (mouse IgG1) and chimeric version (human IgG1) have been tested in various experimental settings to assess the antibody's antitumor effects. In vivo studies demonstrated significant reductions in the growth of the mammary carcinoma cell line F311 and spontaneous lung metastasis in mice vaccinated with a KLH-coupled version of the original antibody. Additionally, intravenous administration of the original antibody to mice inoculated with B16 melanoma cells resulted in a dramatic reduction in lung metastases compared to irrelevant IgG treatment. In vitro, the binding activity of the original version of the antibody was confirmed through ELISA, where the chimeric version of the antibody specifically bound to MAb P3-coated microtiter plates, while a negative control antibody showed no binding (US8758753B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
