Cat. No.
MABL-2606
Application
Catalyzation, crystallization
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
A.17 L47K
From
Recombinant Antibody
Specificity
This antibody is specific for paraoxon.
Alternative Names
L-Leu47Lys; A17 L47K; POX
UniProt
Immunogen
The original antibody was generated via phage display from the Griffin.1 library by panning against reactive phosphonate ester 1.
Application Notes
This antibody is a covalent trap for Paraoxon and other organophosphate substrates, catalyzing the hydrolysis reaction through the SN2 mechanism. The catalytic parameters of this antibody were measured, and its KD was found to be 170 ± 8 μM; it exhibited a 340-fold reactivity enhancement for paraoxon relative to wild-type Ig-paraoxonase. Furthermore, its crystal structure in complex with paraoxon was determined (Mokrushina et al., 2020; PMID: 32859757).
Antibody First Published
Mokrushina et al. Multiscale computation delivers organophosphorus reactivity and stereoselectivity to immunoglobulin scavengers Proc Natl Acad Sci U S A. 2020 Sep 15;117(37):22841-22848. doi: 10.1073/pnas.2010317117. Epub 2020 Aug 28 PMID:32859757
Note on publication
The original publication explores the enhancement of immunoglobulin (Ig) catalytic activity against organophosphorus toxins through quantum mechanics/molecular mechanics (QM/MM) maturation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
