Cat. No.
MABL-2612
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Fish
Clone No.
3E1G12B6
From
Recombinant Antibody
Specificity
This antibody is specific for fish parvalbumin. Its epitope is IKKAFAVID. In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Alternative Names
Parvalbumin; PRV7; 3E1
UniProt
E3TEC5
Immunogen
The mouse version of this antibody was raised by immunizing a mouse with heat-treated catfish sarcoplasmic protein extract.
Application Notes
Both a western blot and an ELISA were performed on fish proteins using the mouse version of this antibody to characterize the binding of this antibody (Gajewski and Hsieh, 2009; pmid:19435232). To study relationships among matrix effect, extractability, and thermostability during in vitro immunodetection, a western blot was performed on both crude fish extract and purified parvalbumin (Keshavarz et al, 2019; pmid:30372974). To analyse the immunochemical properties of pepsin digested tropomyosin peptide fragments from Atlantic salmon and flathead grey mullet, a western blot was performed on purified parvalbumin (Keshavarz et al, 2020).
Antibody First Published
Gajewski and Hsieh Monoclonal antibody specific to a major fish allergen: parvalbumin J Food Prot. 2009 Apr;72(4):818-25. PMID:19435232
Note on publication
An antibody was generated against the fish allergen parvalbumin and further characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
