Cat. No.
MABL-2616
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
LD2 sAB
From
Recombinant Antibody
Specificity
The antibody binds specifically to the LD-2 motif of human paxillin with a Kd of 24 nM.
Alternative Names
PXN
UniProt
P49023
Immunogen
Peptide corresponding to paxillin-LD2 motif (residues 141-156) fused to MBP; peptide sequence: NLSELDRLLLELNAVQHNP.
Application Notes
This antibody binds to the LD2-motif of human paxillin, a signal transduction adaptor protein involved in focal adhesion, with binding partners including integrins, Src, FAK or actin complexes. The antibody inhibits complex formation between paxillin and FAK. Paxillin is involved in both inside-out cell signalling and outside-to-inside cell signalling.
Antibody First Published
Nocula-Lugowska et al. Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin. Journal of Molecular Biology 2015; S0022-2836(15)00344-7 PMID:26087144
Note on publication
Describes the generation and crystallization of two synthetic antibodies against the LD2 and LD4 motifs of human paxillin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
