Cat. No.
MABL-2620
Application
IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Insect, S. pombe, Human
Clone No.
PC10
From
Recombinant Antibody
Specificity
This antibody binds to PCNA from various vertebrate, invertebrate and fungal species.
Alternative Names
Proliferating cell nuclear antigen; cyclin or polymerase delta accessory protein
UniProt
P12004
Immunogen
Protein A-PCNA fusion obtained from pC2T.
Application Notes
PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is potentially a therapeutic target in cancer therapy and a useful marker for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis). This antibody can be used for grading of different neoplasms, e.g. astrocytoma. It can be of diagnostic and prognostic value. Recommended for use on formalin-fixed paraffin embedded sections. NEUTRAL ELUTE ONLY.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
