Cat. No.
MABL-2635
Application
Blocking, inhibition, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6#
From
Recombinant Antibody
Specificity
The antibody is specific for PD-1. PD-1 is a type I membrane protein with 288 amino acids, and is one of the main known immune checkpoints. PD-1 is expressed in activated T lymphocytes, and it binds ligands PD-L1 and PD-L2.
Alternative Names
CD279; Programmed cell death protein 1; PDCD1; PD-1; hPD-1; Human programmed cell death receptor-1
UniProt
Q15116
Immunogen
The original antibody was generated by immunizing BALB/c mice with PD-1.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The original format and the humanized version of the antibody were capable of blocking the binding of PD-1 and PD-L1. The antibody was able to activate the T cells under in vitro culture conditions and promote the production of IFN-γ, thereby enhancing their T cell immune function. The binding affinity of the murine and humanized versions of the antibody to PD-1 were measured by surface plasmon resonance (Kd= 6.74×10 - 10 M and 8.07×10 -10 M respectively). The antibody blocked the interactions between PD-1 and PD-L1. The antibody inhibited tumor growth in NCG mouse models of non-small cell lung cancer and colorectal cancer tumors (CN107043420B).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
