Cat. No.
MABL-2638
Application
Blocking, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
J116
From
Recombinant Antibody
Specificity
This antibody is specific for human PD-1.
Alternative Names
CD279; PD1; PD 1; Programmed cell death protein 1; Programmed cell death 1; pdcd1; Protein PD-1; hPD-1
UniProt
Q15116
Immunogen
The original antibody was raised by immunising BALB/c mice with a human PD-1:IgG1 fusion protein.
Application Notes
In immunohistochemical analysis, this antibody has been used to stain for PD-1 expression in human breast cancer frozen tissue sections (Ghebeh et al, 2008), human tonsil cryosections (Iwai et al, 2002), acetone-fixed liver tissue sections (Wang et al, 2011) and formalin-fixed, paraffin- embedded human gastric neuroendocrine carcinoma tissue sections (Yang et al, 2019). This PD-1 blocking antibody has been used, in combination with an anti-PD-L1 antibody, to assess the effects of PD-1 pathway blockade on tumor-infiltrating lymphocytes (Matsuzaki et al, 2010). It has also been used to block PD-1 in samples of human peripheral blood and pleural fluid mononuclear cells (Jurado et al, 2008), and to test the effects of PD-1 blockade on peripheral blood mononuclear cell proliferation and interferon‐γ production in samples from HIV-1-positive patients (Rosignoli et al, 2009).
Antibody First Published
Iwai et al. Microanatomical localization of PD-1 in human tonsils Immunol Lett. 2002 Oct 1;83(3):215-20 PMID:12095712
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
