Cat. No.
MABL-2643
Application
Blocking, FACS, in vitro, in vivo, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RMP1-30
From
Recombinant Antibody
Specificity
This antibody is specific for mouse PD-1.
Alternative Names
CD279; mPD-1; Protein PD-1; Programmed cell death protein 1
UniProt
Q02242
Immunogen
The original antibody was generated by immunizing rats with mouse PD-1 transfectants.
Application Notes
The original version of this antibody (rat IgG2b) was used to investigate PD-1 signaling pathways in the context of allergic sensitization and challenge in mice. Specifically, it was used to determine how PD-1 blockade affects airway reactivity and immune responses (Matsumoto et al., 2004; PMID: 14764726). The original antibody was used successfully for blocking the PD-1 pathway in combination with ex vivo TKD/IL-2-stimulated NK cells in preclinical models of glioblastoma and lung cancer. It was found that this combination therapy significantly enhanced the cytotoxic activity of NK cells compared to monotherapy. The treatment resulted in improved overall survival, massive infiltration of tumors by CD8+ cytotoxic lymphocytes and NK1.1 cells, and a reduction of PD-1+ immune cells in the tumor microenvironment.
Antibody First Published
Matsumoto et al., B7-DC regulates asthmatic response by an IFN-gamma- dependent mechanism. J Immunol. 2004 Feb 15;172(4):2530-41. PMID:14764726
Note on publication
The original publication investigates the role of B7-DC in regulating asthmatic responses through an IFN-gamma-dependent mechanism, independent of PD-1 signaling, in a murine model of OVA-induced allergic asthma.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
