Cat. No.
MABL-2647
Application
Blocking, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
29E.2A3
From
Recombinant Antibody
Specificity
This antibody binds human PD-L1.
Alternative Names
CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1
UniProt
Q9NZQ7
Immunogen
Application Notes
The original format of the antibody was able to block the interaction between PD-L1 on the 300.19-hPD-L1 cells and B7-1 on the plate and the interaction between PD-L1 on the 300.19-hPD-L1 cells and PD-1 on the plate (Butte et al., 2008; PMID: 18585785). This antibody was used for detection of PD-L1 expressed in 293T cells by flow cytometry (Broos et al. 2019, PMID: 31394834). The humanized version of the antibody was constructed (AU2019201688). Immunohistochemical staining of frozen tissue sections was performed using the antibody to stain thymic epithelia (Brown et al., 2003; PMID: 12538684).
Antibody First Published
Latchman et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation Nat Immunol. 2001 Mar;2(3):261-8. doi: 10.1038/85330. PMID:11224527
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
