Cat. No.
MABL-2650
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AB1
From
Recombinant Antibody
Specificity
This antibody binds human PD-L1.
Alternative Names
CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1
UniProt
Q9NZQ7
Immunogen
The original antibody was generated from a yeast display library by panning against B7-H1-Fc biotinylated protein.
Application Notes
The scFv version of this antibody binds human B7-H1 with an affinity of 2.88 nM. The ability of this antibody to block binding of PD-1-Fc protein or B7.1-Fc protein to B7-H1-Fc was evaluated using ELISA. This antibody could effectively block B7-H1/PD-1 binding and B7-H1/B7.1 binding with an IC50 value of 1.37µg/ml and 0.521.37µg/ml respectively. The ability of the AB1 to bind to human B7-H1, mouse B7-H1 was determined by flow cytometric analysis. This antibody did not exhibit any binding to 293F cells transfected with mouse B7-H1, human PD-1 or human ICOS. This antibody was also shown to IFN-γ release in a PBMC asssay. It was also reported that this antibody can inhibit A375 human melanoma tumor growth in mouse model (WO2015195163).
Antibody First Published
WO2015195163
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
