Cat. No.
MABL-2669
Application
functional assays, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Fungal
Clone No.
B1
From
Recombinant Antibody
Specificity
This antibody binds to pectinases from various pathogenic fungi from such groups as ascomycetes, basidiomycetes, and oomycete.
Alternative Names
Polygalacturonase; PG; Endopolygalacturonase; ScFvB1; AY741402; enzyme pectinase
UniProt
Immunogen
Spleen mRNA from mice immunized with extracellular proteins from Rhizoctonia solani was used to construct a cDNA library from which antipectinase ScFv antibodies were isolated using phage display techniques (Manatunga et al., 2005).
Application Notes
B1 scFV antibody was shown to inhibit polygalacturonase (PG) activity in a number of pathogenic species of fungi and thus, it is a highly promising agent in the research on the methods of plant fungal diseases prevention (Manatunga et al., 2005). Furthermore, this antibody was also able to inhibit maceration caused by infection of plant tissue by necrotrophic fungi (Manatunga et al., 2005).
Antibody First Published
Manatunga et al. Maceration of plant tissue by fungi is inhibited by recombinant antipectinaseantibodies European Journal of Plant Pathology (2005) 112: 211–220
Note on publication
This article describes the generation and characterisation of the B1 scFv.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
