Cat. No.
MABL-2691
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Trimeresurus stejnegeri (Chinese green tree viper, Viridovipera stejnegeri)
Clone No.
PLA 2-mc8
From
Recombinant Antibody
Specificity
This antibody reacts specifically with phospholipase A2 (PLA2) protein present in the venom of Trimeresurus stejnegeri. PLA2 inhibits platelet aggregation induced by ADP, arachidonic acid and PAF. It acts in an enzymatic independent manner on a proteinase-activated receptor (PAR1, F2R) to evoke calcium release through the inositol 1,4,5-trisphosphate receptor (ITPR1, IP3R) and induces mouse aorta contraction.
Alternative Names
PLA2; Snake venom phospholipase A2; Phospholipase A2 5
UniProt
P82896
Immunogen
The original antibody was generated by immunizing BALB/c mice with natural phospholipase A2 protein purified from crude venom of Trimeresurus stejnegeri.
Application Notes
This antibody can be used for the detection of PLA2 protein of Trimeresurus stejnegeri by ELISA. The binding specifcity of the antibody was further confirmed by detection of the protein in a western blot (CN113930408; PMID: 34899734).
Antibody First Published
Long et al. A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites. Front Immunol. 2021 Nov 9:12:775678. PMID:34899734
Note on publication
Describes the generation of monoclonal and polyclonal antibodies against SVMP protein and PLA2 protein found in snake venom.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
