Cat. No.
MABL-2695
Application
in vitro, in vivo, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
C. burnetii
Clone No.
1E 4
From
Recombinant Antibody
Specificity
The antibody recognizes a PI specific epitope on PI-LPS. The antibody does not cross react with PII-LPS.
Alternative Names
phase I lipopolysaccharide; LPS phase I; C. burnetii PI-LPS; C. burnetii phase I lipopolysaccharide
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with formalin-inactivated C. burnetii Nine Mile PI antigen.
Application Notes
The specificity of the antibody (IgG2a) for PI-LPS was confirmed by ELISA analysis. The antibody detected PI-LPS by western blot analysis. The antibody was able to inhibit C. burnetii infection in vivo in a dose-dependent manner (Peng et al., 2012; PMID: 23053512). The original antibody could confer protection against C. burnetii aerosol infection. The Fab fragment, the scFv fragment and the humanized version of the antibody were constructed and characterized by indirect ELISA and Western Blot. They were able to bind to C. burnetii and to inhibit the infection in mice and in mouse Bone Marrow-Derived Macrophages (BMDM) in vitro. The humanized version inhibited C. burnetii infection in human macrophages in vitro. The Fab fragment was able to neutralize virulent C. burnetii resulting in inhibiting C. burnetii infection in both in vitro and in vivo systems (US20150087807A1).
Antibody First Published
Peng et al. Development of a lipopolysaccharide targeted peptide mimic vaccine against Q fever. J Immunol. 2012 Nov 15; 189(10): 10.4049/jimmunol.1201622. PMID:23053512
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
