Cat. No.
MABL-2702
Application
IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
10B2
From
Recombinant Antibody
Specificity
This antibody recognizes Plakophilin-1. It binds the epitope within the first 27 amino acids from the N-terminal region and it does not cross react with Plakophilin 2 and Plakophilin 3. Plakophilins influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilin-1 is thought to play a role in junctional plaques and contribute to epidermal morphogenesis.
Alternative Names
PKP1; PKP-1; Plakophilin-1; Band 6 protein; B6P; PKP 1
UniProt
Q13835
Immunogen
This antibody was raised by immunizing mice with the N-terminus of human Plakophilin-1 protein.
Application Notes
10B2 is recommedned for the detection and analysis of Plakophilin-1. In order to identify potential perturbations in cell adherence, this antibody was used to detect plakophilin-1 in the skin tissue of patients with epidermolysis bullosa via immunofluorescence (Pigors et al., 2011; pmid: 21320868). Further, this antibody was demonstrated to have a potential diagnostic role in certain diseases, such as Ectodermal Dysplasia/Skin Fragility Syndrome where it was used to differentiate between a diseased and healthy skin samples (Sprecher et al., 2004; pmid: 15086548). It was also shown that 10B2 antibody can detect plakophilin-1 (PKP1) via Western blot (here: in HaCaT protein lysates) (Bonne et al., 2003; pmid: 12707304).
Antibody First Published
Bonne et al. Defining desmosomal plakophilin-3 interactions J Cell Biol. 2003 Apr 28;161(2):403-16. doi:10.1083/jcb.200303036. Epub 2003 Apr 21. PMID:12707304
Note on publication
This article describes the earliest characterization and usage of this anti-plakophilin 1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
