Cat. No.
MABL-2709
Application
WB, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mAb3D3
From
Recombinant Antibody
Specificity
The antibody is specific for human podocalyxin. The epitope recognized by the antibody is located on the extracellular domain of podocalyxin. Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes in the glomerulus (Bowman's capsule) in the kidneys. It is a member of the CD34 family of transmembrane sialomucins.
Alternative Names
PC; PCLP-1; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; 3D3
UniProt
O00592
Immunogen
The original antibody was generated by immunizing BALB/C mice with purified human podocalyxin-glutathione S-transferase (GST) fusion protein containing the intracellular, transmembrane, and a part of the extracellular domain of human PCLP.
Application Notes
The antibody recognized rabbit PCLP1 in human renal cortical sections on immunofluorescence. The antibody detected PCLP by western blot analysis (Kershaw et al., 1997; PMID: 9188463). The antibody detected PCLP in HEV of tonsil by immunofluorescence analysis. The antibody immunoprecipitated PCLP from colon carcinoma cell lysate (Thomas et al., 2009; PMID: 19118161).
Antibody First Published
Kershaw et al. Molecular cloning and characterization of human podocalyxin- like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin. J Biol Chem. 1997 Jun 20;272(25):15708-14. doi: 10.1074/jbc.272.25.15708. PMID:9188463
Note on publication
The original paper describes the generation and characterization of the original antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
