Cat. No.
MABL-2711
Application
WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Elephant
Clone No.
Pmab-295
From
Recombinant Antibody
Specificity
This antibody is specific for elephant Podoplanin (elePDPN). PDPN is an essential marker of lung type I alveolar cells, kidney podocytes, and lymphatic endothelial cells.
Alternative Names
PDPN; elePDPN; Elephant PDPN; Elephant podoplanin
UniProt
G3T5I4
Immunogen
The original antibody was raised by immunizing BALB/c mice four times with three keyhole limpet hemocyanin-conjugated elePDPN peptides, including 38-EGGMVIPGVEDNMV-51, + C-terminal cysteine, followed by a final booster shot.
Application Notes
This antibody recognized both elePDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells and endogenous elePDPN-expressing LACF-NaNaI cells by flow cytometry (FC) and Western blotting (WB). Kinetic analyses using FC showed that this antibody's KD for CHO/elePDPN was 1.5 × 10-8 M. Furthermore, this antibody detected elePDPN-expressing cells using immunohistochemistry (IHC) (Kudo et al., 2022; PMID: 35917562). An ELISA with the alanine-substituted peptides of the elePDPN extracellular domain (amino acids 38–51) revealed that the binding epitope of this antibody includes Met41, Pro44, and Glu47 of elePDPN (Okada et al., 2022; PMID: 35917553).
Antibody First Published
Kudo et al. Development of a Monoclonal Antibody PMab-295 Against Elephant Podoplanin Monoclon Antib Immunodiagn Immunother. 2022 Aug;41(4):194-201. doi: 10.1089/mab.2022.0007 PMID:35917562
Note on publication
The original publication describes the development and characterization of a novel monoclonal antibody, PMab-295, that specifically recognizes elephant podoplanin (elePDPN).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
