Cat. No.
MABL-2794
Application
FACS, IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4E3/1
From
Recombinant Antibody
Specificity
This antibody recognises human Rad54. Rad54 is a protein engaged in recombination during mitosis as well as DNA repair mechanisms.
Alternative Names
DNA repair and recombination protein RAD54-like; RAD54 homolog; hHR54; hRAD54; RAD54ADNA repair and recombination protein RAD54-like; RAD54-like (S. cerevisiae); hRAD54HRAD54; HR54; EC 3.6.1; hHR54EC 3.6.4.-; RAD54 like
UniProt
Q92698
Immunogen
This antibody was raised against full length human Rad54 protein.
Application Notes
IF and IP: This antibody was used in an immunoprecipitation experiment and to investigate colocalisation of Rad54 with S100A11 via immunofluorescence in fixed HaCaT cells treated with BLM (Murzik et al., 2008). WB and IF: Rad51 was visualised both on Western blots and immunofluorescently in BS/A-15 and NHF cells with this anti-Rad54 antibody (Srivastava et al., 2009).
Antibody First Published
Murzik et al. Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11. Mol Biol Cell. 2008 Jul;19(7):2926-35. doi: 10.1091/mbc.E07-11- 1167. Epub 2008 May 7. PMID:18463164
Note on publication
This article describes the earliest identified use of the anti-Rad54 Rad54 4E3/1 antibody. It was used to investigate the potential interaction and colocalisation between Rad54 and another protein (IP and IF experiments, supplemental materials of the article).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
