Cat. No.
MABL-2819
Application
WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Monkey, Rabbit, Human
Clone No.
RNL-2
From
Recombinant Antibody
Specificity
The antibody binds Reticulon-1A/B / NSP-A/B. The antibody recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B).
Alternative Names
Reticulon-1; Neuroendocrine-specific protein; NSP-A/B
UniProt
Q16799
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with the small cell lung cancer cell line NCI-H82.
Application Notes
This antibody was used for immunohistochemistry of NSP-A on squamous cell carcinoma (Senden et al., 1997; PMID: 9227337). The antibody detected NSP-A and B from NCI-H82 cells by western blot analysis. Immunofluorescence using the antibody revealed that NSPs are localized in the endoplasmic reticulum (ER) of NCI-H524 cells and migrated to the ER of COS-1 cells after transfection with the NSP-B construct. The antibody recognized a sub-set of neuro-endocrine tissues and tumours by immunohystochemistry. In normal tissues, the antibody reacted with brain Purkinje cells, pancreatic islet cells, some cells in the pituitary gland and some (peripheral) nerve fibres. Further, spleen reticular fibres, prostatic epithelium and some suprabasal cells of the tongue epithelium were also positive with the antibody.
Antibody First Published
Broers et al. Novel antigens characteristic of neuroendocrine malignancies Cancer. 1991 Feb 1;67(3):619-33. PMID:1845952
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
