Cat. No.
MABL-2821
Application
WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Swine, Human
Clone No.
RNL-4
From
Recombinant Antibody
Specificity
The antibody is specific for reticulon-1C. It recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C).
Alternative Names
NSP-C; Reticulon-1; Neuroendocrine-specific protein
UniProt
Q16799
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
Application Notes
The specificity of the original format of the antibody for NSP-C was established in an ELISA assay. Immunofluorescence analysis of COS-1 cells, transfected with NSP-C cDNA, showed staining of the endoplasmic reticulum with the antibody. The antibody detected NSP-C by western blot analysis (Senden et al., 1996; PMID: 8900485). This antibody was used for immunohistochemistry for the detection of NSP-C on lung carcinoid, atypical carcinoid of the lung, SCLC and squamous cell carcinoma (Senden et al., 1997; PMID: 9227337).
Antibody First Published
Senden et al. Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A Eur J Cell Biol. 1996 Mar;69(3):197-213. PMID:8900485
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
