Cat. No.
MABL-2824
Application
ADCC, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D7C2
From
Recombinant Antibody
Specificity
Alternative Names
CD240D; RHXIII; Rh polypeptide 2; RhPII
UniProt
Q02161
Immunogen
A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Application Notes
FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).
Antibody First Published
Edelman et al, 1997. Obtaining a functional recombinant anti-rhesus (D) antibody using the baculovirus-insect cell expression system. Immunology. 1997 May; 91(1): 13–19. PMID:9203960
Note on publication
Describes the original production of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
