Cat. No.
MABL-2831
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
1F3-B3-E5
From
Recombinant Antibody
Specificity
The antibody recognizes human, mouse, and rat RhoG. The antibody does not cross react with purified RhoA or Cdc42. RhoG (ARGH) is a member of the Ras super-family of small G-proteins and a member of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The GEF displaces GDP from the binding site of RhoG and allows GTP to bind in its place rendering the protein active. This active RhoG binds downstream effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to GDP making it inactive. RhoG is believed to be crucial for integrin-mediated Rac activation and cell spreading. RhoG is believed to be active upstream of Rac and work independently of Rac.
Alternative Names
Rho-related GTP-binding protein RhoG, Ras homology Growth-related, ARHG
UniProt
P84095
Immunogen
The antibody was raised by immunizing A/J mice with amino acids 161-180 of RhoG protein conjugated to KLH.
Application Notes
The specificity of this antibody was confirmed by ELISA analysis. The antibody recognized RhoG on Western blots but it did not recognize Rac1 or Cdc42 (Meller et al, 2008; pmid:18505794). Immunofluorescence was performed on MDA-MB-231 cells using this antibody (Yamaki et al, 2010; pmid:20679435). Immunofluorescence was also performed on COS-7 cells (Tzircotis et al, 2011; pmid:21878497).
Antibody First Published
Meller et al. Endogenous RhoG is dispensable for integrin-mediated cell spreading but contributes to Rac-independent migration. J Cell Sci. 2008 Jun 15;121(Pt 12):1981-9. PMID:18505794
Note on publication
The paper describes the generation and characterization of the antibody, which is specific for RhoG.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
