Cat. No.
MABL-2846
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ROS1-5F2
From
Recombinant Antibody
Specificity
This antibody binds ROS1 kinase domain. Mutations, rearrangements, and overexpression of the ROS1 gene can all lead to the imbalance of the ROS1 kinase protein, and the abnormal ROS1 protein kinase activity activates multiple downstream oncogenic signal pathways, including PI3K/AKT/mTOR, STAT3, RAS-MAPK/ERK, VAV3 and SHP-1/2 etc.
Alternative Names
MCF3, ROS; Proto-oncogene tyrosine-protein kinase ROS; Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase
UniProt
P08922
Immunogen
The original antibody was generated by immunizing mice intramuscularly with purified peptide 'GGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRK and immune enhancer PEI in a ratio of 1:3 and dissolved in a serum free and dual-antibody-free DMEM solution. The purified peptide is identified as a specific binding fragment of PF-06463922 (Crizotinib, Xalkori).
Application Notes
The binding specificity of this antibody for ROS1 was determined using ELISA. This antibody was also capable of detection the ROS1 kinase domain polypeptide in a western blot assay. The original IgG2b version of this antibody was capable of binding the antigen with a high affinity and the dissociation constant reaches Kd=3.56×10-11M. The affinity measurement was done using surface plasmon resonance. This antibody showed a good inhibitory effect on hepatocellular carcinoma cells (HepG2) in a CCK-8 cytotoxicity test (CN111440241).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
