Cat. No.
MABL-2860
Application
crystallization
Isotype
Engineer antibody
Species Reactivity
Toxoplasma gondii
Clone No.
4F11E12
From
Recombinant Antibody
Specificity
4F11E12 recognises a highly conserved conformational epitope on the surface of the D1 domain in both native and recombinant SAG1, located within the N-terminal domain; the binding site does not overlap with the proposed ligand-binding pocket or homodimerization site. The eptiope is an immunodominant area and includes residues from 2 long loops located at one exteremity. SAG1 is a GPI- anchored cell surface protein predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. SAG1 recognizes ligands such as glucosamide moieties, which are expressed in a broad range of host cell types. T. gondii is a protozoan parasite capable of causing chronic infections resulting in congenital disease and has the ability to evade natural host defences.
Alternative Names
P30
UniProt
C7E5U4
Immunogen
4F11E12 was generated by intraperitoneal infection of mice with cysts from the 76 K T. gondii strain and was selected for its ability to inhibit efficiently the recognition of SAG1 by sera from infected patients. The 4F11E12 Fab fragment was created by digestion with papain
Application Notes
F411E12 can be co-crystallized with SAG1, which enables the mode of binding to be elucidated.
Antibody First Published
Graille et al. Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune response. J Mol Biol. 2005 Nov 25;354(2):447-58. Epub 2005 Sep 30. PMID:16242717
Note on publication
Describes the generation of F411E12 Fab and its co-crystallization with SAG1. The epitope binding site was determined and it was found that netiher homodimerization or prevents access to the ligand binding site (which are necessary to neutralize parasite entry into host cells).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
