Cat. No.
MABL-2878
Application
ELISA, WB, Inhibit
Isotype
Engineer antibody
Species Reactivity
Staphylococcus aureus
Clone No.
14G8
From
Recombinant Antibody
Specificity
This antibody binds a conformational epitope near the C terminal end of the Staphylococcal enterotoxin B. This antibody is suspected to interact with residues 135-R, 137-F, 186-Y, 188-K, 231-E, 233-Y, and 235, 236-T.
Alternative Names
Staphylococcal enterotoxin B; Enterotoxin type B; entB
UniProt
P01552
Immunogen
The original antibody was generated by immunizing BALB/c mice with full-length staphylococcal enterotoxin B (SEB).
Application Notes
This antibody in combination with 6D3 and 20B1 can be used to detect Staphylococcal enterotoxin B (SEB) in a sample using capture ELISA. This antibody is capable of detecting SEB in a western blot assay but not staphylococcal enterotoxin A (SEA) or toxic shock syndrome toxin 1 (TSST-1). 14G8 demonstrated comparable level of inhibition of SEB incuded T cell-proliferation in human T-cells from a normal donor. 14G8 also caused inhibition of cytokine induction in treated T-cells as they produced less IFN- gamma and IL-2. It was reported that 14G8 alone did not provide any protection against SEB induced lethal shock (SEBILS) in BALB/c mice and HLA-DR3 transgenic mouse model. However, when used in combination with mAb 6D3 or 20B1 it showed better results (PMID: 21233204).
Antibody First Published
Varshney et al. Generation, characterization, and epitope mapping of neutralizing and protective monoclonal antibodies against staphylococcal enterotoxin b-induced lethal shock. J Biol Chem. (2011); 286(11): 9737–9747. PMID:21233204
Note on publication
Describes the generation, characterization and epitope mapping of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
