Cat. No.
MABL-2880
Application
SPR, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Staphylococcus aureus
Clone No.
4PCL2
From
Recombinant Antibody
Specificity
This antibody recognizes and binds Staphylococcal enterotoxin B.
Alternative Names
Staphylococcal enterotoxin B; Enterotoxin type B; entB; 2F6D9G2
UniProt
P01552
Immunogen
The original antibody was generated by immunizing eight-week-old female BALB/c mice were immunized subcutaneously with SEB at 3-week intervals. Later on a phage display library was generated from the variable regions of the hybridoma and this clone was isolated by panning against SEB.
Application Notes
This antibody can be used for the immundection of SEB in a sample. This antibody was derived from the murine parent antibody called 2F6D9G2, after PCR cloning the variable region genes of the hybridoma to generate a phage display library and isolating the specific clone that showed reactivity towards SEB. The binding reactivity of the scFv clone towards SEB was determined using ELISA. The scFv clone binds SEB with a high affinity of 3.16 nM as determined by surface plasmon resonance (SPR). This scFv antibody was also capable of detecting SEB in a western blot assay (PMID: 20952642).
Antibody First Published
Singh et al. Construction of a single-chain variable-fragment antibody against the superantigen Staphylococcal enterotoxin B. Appl Environ Microbiol. 2010 Dec;76(24):8184-91. PMID:20952642
Note on publication
Describes the generation of an antibody against Staphylococcal enterotoxin B and further describes the generation of a murine phage display library containing single-chain variable-fragment (ScFv) antibody having binding affinity against SEB.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
