Cat. No.
MABL-2884
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3B8-H3-G1
From
Recombinant Antibody
Specificity
This antibody is specific for human serum albumin. Albumin binds water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood.
Alternative Names
HSA-1; Albumin; ALB
UniProt
Immunogen
The mouse version of this antibody was raised by immunizing a BALB/c mouse with human serum albumin.
Application Notes
To create an assay which can measure albumin, an ELISA was performed on human albumin using the mouse version of this antibody (Benjamin et al, 1987; pmid:2437011).
Antibody First Published
Benjamin et al. A unique epitope on human serum albumin recognized by monoclonal antibody HSA-1: a probe for identification of the human origin of blood or tissue Hybridoma. 1987 Apr;6(2):183-90. PMID:2437011
Note on publication
A double antibody ELISA assay was developed using biotinylated HSA-1 as the specific probe for human albumin. This assay was capable of detecting as little as 30 nanograms or less albumin/ml. This assay was used to verify the presence of human albumin in blood, tissue extracts, and other body fluids.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
