Cat. No.
MABL-2894
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Aequorea victoria (Water jellyfish, Mesonema victoria)
Clone No.
NbsfGFP01
From
Recombinant Antibody
Specificity
This antibody is specific for superfolder green fluorescent protein which is a better-folding GFP version, with the following mutations: S30R, Y39N, F64L, S65T, F99S, N105T, Y145F, M153T, V163A, I171V and A206V.
Alternative Names
Superfolder green fluorescent protein; GFP; Green fluorescent protein
UniProt
P42212
Immunogen
The original antibody was generated by immunizing an adult one-humped camel with recombinant superfolder green fluorescent protein (sfGFP). An immune nanobody library was generated from the peripheral blood of the immunized camel. The particular antibody was isolated by panning the library against sfGFP.
Application Notes
The binding specificity of this nanobody towards sfGFP was determined using ELISA. This antibody can also be used of the identification of sfGFP via a western blot (PMID: 25085037).
Antibody First Published
Twair et al. Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Mol Biol Rep. 2014 Oct;41(10):6887-98. PMID:25085037
Note on publication
Describes the generation of camelid nanobodies against superfolder GFP.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
