Cat. No.
MABL-2936
Application
ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Chicken, Feline, Monkey, Swine, Human
Clone No.
R4A
From
Recombinant Antibody
Specificity
The antibody reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation.
Alternative Names
UniProt
P53814
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with a cytoskeletal extract of chicken gizzard.
Application Notes
The antibody detected Smoothelin by western blot analysis. This antibody was used for immunohistochemical screening of different species and tissues (i.e., intestinal smooth muscle tissue of human, goat, Xenopus laevis, and chicken oesophagus, human large intestine, stomach, uterus, and prostate, smooth muscle cells) for the presence of smoothelin. COS7 cells transfected with the full-size human smoothelin cDNA showed filamentous structures that were reactive with the antibody in immunofluorescence analysis (van der Loop et al., 1996; PMID: 8707825). This antibody was used for immunohistochemistry on normal smooth muscle (Coco et al., 2009; PMID: 19950405).
Antibody First Published
van der Loop et al. Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells J Cell Biol. 1996 Jul;134(2):401-11. PMID:8707825
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
