Cat. No.
MABL-3000
Application
Block
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D1 (A12)
From
Recombinant Antibody
Specificity
This antibody binds human (not mouse) TACE catalytic and non-catalytic domains.
Alternative Names
ADAM17; tumor necrosis factor-α-converting enzyme; CD156b
UniProt
P78536
Immunogen
Recombinant human ADAM17 (TACE) ectodomain tagged to biotin.
Application Notes
TACE (CD156b) has proved capable of cleaving epidermal growth factor receptor (EGFR) ligands, extracellular Notch1, cell-surface receptors, and adhesion molecules. As proteolytic cleavage is an indispensable activation event for many of these substrates, TACE has emerged as an attractive therapeutic target for the treatment of cancer and rheumatoid arthritis. This “cross-domain” human antibody is a selective TACE antagonist and provides a unique alternative to smallmolecule metalloprotease inhibition. Note from inventor: "D1(A12) is conformation sensitive and sees only a specific form of the native enzyme (reflecting its redox state). It does not work by IHC or WB. It was designed as a potential drug, active in native situations".
Antibody First Published
Tape et al. Cross-domain inhibition of TACE ectodomain Proc Natl Acad Sci U S A. 108(14):5578-83 PMID:21415364
Note on publication
Describes the generation and characterisation of this antibody by phage-display.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
