Cat. No.
MABL-3014
Application
FACS, IP, Block, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BMA031
From
Recombinant Antibody
Specificity
This antibody reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. TCR-αβ is expressed by the majority of mature CD3+ T cells.
Alternative Names
Human α/β T Cell Receptor (TCR)
UniProt
Immunogen
The original antibody was generated by immunizing mice with human perihperal blood T- lymphocytes.
Application Notes
This antibody is recomended for detection of viable human αβ+ TCR lymphocyte. It is highly effective in modulating immune reactions and is capable of activating human T lymphocytes in vito (PMID: 3092794). Double immunofluorescence staining was used to ascertain that this antibody does not cross react with γδ+ TCR cells. FACS analysis confirmed that BMA031 did not react with γδ+ TCR cells. BMA031 successfully precipitates TCR αβ/CDR complex (PMID: 1704361). BMA031 in conjugation with triple drug induction treatment in kidney-transplanted patients showed no side effects and reduction in graft rejections (PMID: 2978369). A clinical phase II trial administered 50 mg of murine anti-human alpha/beta- TCR mAb (BMA 031) intravenously on the day of, as well as 2 and 4 days after, cadaveric donor renal transplantation in combination with a CsA/prednisone regimen. After a minimum of 30 months follow-up, the actual allograft survival rate was 87% in the BMA-treated group compared with 68% in the control cohort (PMID: 8009591).Humanized version of this antibody BMA 031-EUCIV3 is found to have 2.5 times less affinity than its murine parent, however the ability to cause antibody dependent cell-mediated cytolysis was significantly enhanced (PMID: 1836485).
Antibody First Published
Kurrle et al. Distinction of two CD3-monoclonal antibodies in respect to the effectivity to modulate the induction of IgM- and gamma-interferon synthesis. Behring Institute Mitteilungen (1986), (80):48-58. PMID:3092794
Note on publication
Describes the role of this antibody as a tool for analysing mechanisms of T cell activation, interaction between T-cells, B-cells, accessory cells and intermediating immune factors.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
