Key features and details | |
Cat. No. | MABL-1047 |
Name | Anti-CD59 mAbs |
Clone No. | AFD- 193-27 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes human CD59 which is a glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. It regulates complement-mediated cell lysis, and is involved in lymphocyte signal transduction. |
Alternative Name | 1F5; 1F5 antigen; CD59 glycoprotein; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin |
UniProt | P13987 |
Immunogen | This antibody was raised by immunizng mice with stimulated human leukocytes. |
Application Notes | The antibody 193-27 is recommended for the identification and analysis of CD59 via various experimental techniques (Bellosillo et al., 2001). Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH) and thus this antibody is useful for study on GPI-anchored proteins, PNH and CD59 functions. |
Antibody First Published | Bellosillo et al Complement-mediated cell death induced by rituximab in B- cell lymphoproliferative disorders is mediated in vitro by a caspase-independent mechanism involving the generation of reactive oxygen species. Blood. 2001 Nov 1;98(9):2771-7. PMID:11675350 |
Note on publication | This article describes the use of the antibody 161-2. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
