Key features and details | |
Cat. No. | MABL-1046 |
Name | Anti-CD56 ANK-1 mAbs |
Clone No. | AFD- 8G6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC (P), WB, FC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody binds an epitope called ANK-1 present on most NCAM isoforms expressed on a subset of NK cells of mouse and human. This epitope is selectively expressed on pre-activated, adherent natural killer NK cells (pre-A-NK cells), which are interleukin (IL)-2-stimulated NK cells, that are selectively endowed with the increased expression of integrins and ability to adhere to solid surfaces, migrate into, infiltrate, and destroy cancerous tissues. |
Alternative Name | CD56; ANK1; NCAM1; NCAM-1; N-CAM-1; Ncam; Neural cell adhesion molecule 1 |
UniProt | P13595 |
Immunogen | The original antibody was generated by immunizing rats with purified mouse brain N-CAM. |
Application Notes | This antibody was used to classify the nonactivated human peripheral blood NK cells expressing ANK-1 epitope using flow cytometry. It was reported that ANK-1 epitope was preferentially expressed by around 26% of NK cells (range 9–56%) and was exclusively CD56dim. This antibody was also capable of detecting ANK-1 epitope from crude human peripheral blood NK cell and affinity purified brain N- CAM lysates using western blot. This antibody was also used to test the ability of NK cells to respond to IL-2 by generation and expansion of A-NK cells with LAK activity, following depletion of ANK-1+ NK cells by treatment with mAb ANK-1 and complement. This antibody can also be used for the immunohistochemical staining of paraformaldehyde fixed paraffin embedded tissues of spleen, lymph nodes, tonsils, intestines, lungs, and liver tissues (PMID: 15356097). |
Antibody First Published | Shen Li et al. A novel epitope of N-CAM defines precursors of human adherent NK cells. J Leukoc Biol. 2004 Dec;76(6):1187-99. PMID:15356097 |
Note on publication | This paper studies a subpopulation of NK cells expressing a unique epitope ANK-1 using an antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
Key features and details | |
Cat. No. | MABL-1045 |
Name | Anti-CD56 mAbs |
Clone No. | AFD- RNL-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CD56. |
Alternative Name | N-CAM; N-CAM-1; NCAM-1; Neuronal Cell Adhesion Molecule; Neural cell adhesion molecule 1 |
UniProt | P13591 |
Immunogen | The original antibody was generated by using a NCI-H82 cell preparation as an immunogen. |
Application Notes | The original antibody stained CD56 in NCI-H82 cells (Broers et al., 1991; PMID: 1845952). The antibody was reactive with SCLC, lung carcinoids, renal cancers, and some sarcomas. The antibody was reactive in normal neuronal tissues, and endocrine glands, such as pancreatic islet cells, pituitary gland, and adrenal medulla. The antibody reacted positively in the immunofluorescence assay with the NCAM-transfected 3T3 cells (Boerman et al., 1991; PMID: 1645701). The antibody was used to detect CD56 on SCLC cell lines by immunohistochemistry (Borghtet al., 2018; PMID: 30050775). |
Antibody First Published | Broers et al. Novel antigens characteristic of neuroendocrine malignancies Cancer. 1991 Feb 1;67(3):619-33. PMID:1845952 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
