Cat. No.
MABL-3040
Application
functional assay, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Clostridium tetani
Clone No.
9F12
From
Recombinant Antibody
Specificity
This antibody binds the intact or wild type tetanus toxin of Clostridium tetani. It was also reported to bind the tetanus toxin fragment B. Tetanus toxin is an extremely potent neurotoxin produced by Clostridium tetani.
Alternative Names
TT; TTx; Tetanus toxin; tetX; Tentoxylysin
UniProt
P04958
Immunogen
The original antibody was generated from the peripheral blood mononuclear cells form human donors after immunization with tetanus toxoids and fusing these lymphocytes with mouse myeloma line P3-X63-Ag 8.653.
Application Notes
The screening of hybridoma supernatants to identify binding towards tetanus toxin was done using ELISA. The protective capacity of this antibody against tetanus toxoid was tested using the mouse tetanus toxin neutralization assay. The mice which received the tetanus toxin preincubated with 9F12 were completely protected (PMID: 7174795). The original antibody was used to study the role of gangliosides in the binding and internalization of bacterial toxins (PMID: 11093135).
Antibody First Published
Gigliotti et al. Protective human hybridoma antibody to tetanus toxin. J Clin Invest. 1982 Dec;70(6):1306-9. PMID:7174795
Note on publication
Describes the generation and neutralization capacity of this antibody towards tetanus toxin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
