Cat. No.
MABL-3055
Application
crystallization, functional assays, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
T3
From
Recombinant Antibody
Specificity
This antibody is specific for Δ9-THC and its metabolites, including 11-nor-9-carboxy-Δ9- tetrahydrocannabinol (11-nor-Δ9-THC—COOH) and 11‑hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC).
Alternative Names
Δ9-THC
UniProt
Immunogen
The original antibody was generated by immunizing mice a THC-BSA conjugate.
Application Notes
The binding affinity and specificity of the original version of this antibody (mouse Fab) were determined in a competitive ELISA. Structural analysis of this antibody — free and in complex with THC — was determined through crystallization. This antibody was also used to develop a sensitive, one- step, homogeneous FRET immunoassay for detecting THC and its metabolites in saliva samples (Niemi et al., 2010; PMID: 20630472) (US8518653B2). This antibody was successfully transiently expressed in N. benthamiana by using a geminiviral expression system. The plant-produced version of this antibody retained the binding activity to Δ9-THC and its metabolites and provided high sensitivity in detecting Δ9- THC in marijuana, inflorescence, and leaf extracts but not in stem, root, or non-cannabis controls (Boonyayothin et al., 2022; PMID: 35686006).
Antibody First Published
Niemi et al., A structural insight into the molecular recognition of a (-)- Delta9-tetrahydrocannabinol and the development of a sensitive, one-step, homogeneous immunocomplex- based assay for its detection. J Mol Biol. 2010 Jul 23;400(4):803-14. doi: 10.1016/j.jmb.2010.05.048. PMID:20630472
Note on publication
The original publication presents the structural analysis of the T3 Fab fragment's interaction with THC and its metabolites.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
