Cat. No.
MABL-3063
Application
cyrstallization, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TN1
From
Recombinant Antibody
Specificity
TN1 binds specifically to TPO at an epitope which consists of residues from the C-terminal end of helix C (R98) and the C–D crossover loop (residues 102–115) and residues from the C-terminal half of helix B (residues 57–61) (PMID: 14769915). TPO is a heavily glycosylated cytokine which acts as the ligand for the hematopoietic receptor c-Mpl. The primary role of TPO is as a regulator of megakaryocytopoiesis and platelet production. Stimulated c-Mpl transduces signals into target cells via the JAK/STAT, Ras and MAPK pathways.
Alternative Names
C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; TPO
UniProt
P40225
Immunogen
TN1 was prepared by immunizing BALB/c mice by intraperitoneal injecction of recombinant human TPO. The Fab fragment was produced via incubation with papain which cleaves at the hinge region of the IgG to remove the Fc region. The product is then subsequently incubated under reducing conditions and the free Cys residue blocked with iodoacetamide.
Application Notes
TN1 can neutralize the biological activity of TPO and can be used to detect TPO in ELISA studies. The Fab fragement of TN1 can also be used to aid the crystallisation of TPO by increasing the area upon which stable crystal contacts can be made (PMID: 11976502).
Antibody First Published
Tahara et al. A sensitive sandwich ELISA for measuring thrombopoietin in human serum: serum thrombopoietin levels in heathy volunteers and in pathients with haemopoietic disorders. British Journal of Haematology 1996, 93, 783-788
Note on publication
Describes the use of TN1 in the development of a reproducible, highly sensitive and specific human TPO ELISA procedure
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
