Cat. No.
MABL-3065
Application
Depletion, IP, FC
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
YKIX 337.217
From
Recombinant Antibody
Specificity
YKIX 337.217 recognises Thy1 cell surface antigen which is a cell adhesion molecule expressed on thymocytes, peripheral T cells, some B cells, monocytes, and granulocytes. This protein is also expressed on cells in the bone marrow, brain, and kidney, as well as on mesenchymal stem cells.
Alternative Names
CD90; Thy1
UniProt
Q9XT67
Immunogen
YKIX 337.217 was prepared by immunizing rats with Con A stimulated canine T cells.
Application Notes
YKIX 337.217 can be used as a marker for canine T-cells in peripheral blood (note that it has been shown that there is high expression on monocytes, and a weaker expression on granulocytes). The antibody also leads to depletion of circulating T cells (PMID： 8252344). This antibody can also be used for IP studies.
Antibody First Published
Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens:summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414
Note on publication
Describes the binding specificity and characterisation (by IF staining and FACS, and IP) of antibodies against canine equivalents of human CD antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
