Cat. No.
MABL-3070
Application
competition assays, RIA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
WR1.7
From
Recombinant Antibody
Specificity
This antibody specifically targets human TPO, a cell surface enzyme critical for thyroid hormone synthesis. TPO is found in nearly all patients with active autoimmune thyroid disease.
Alternative Names
TPO
UniProt
P07202
Immunogen
A cDNA library was constructed from the thyroid tissue of patients with Grave's disease, and antibody fragments were expressed in XL1-Blue cells.
Application Notes
The original form of this antibody (human IgG1) binds human TPO with a Kd of 0.29 nM (Chazenbalk et al., 1993; PMID: 7686925). Competition assays revealed that this antibody binds a distinct epitope compared to clone TR1.9 (Chazenbalk et al., 1993; PMID: 7686925). Further competition studies demonstrated that this antibody inhibits TPO autoantibodies in patients' sera by interacting with serum TPO autoantibodies (Chazenbalk et al., 1993; PMID: 7505290). Alongside clones TR1.9, SP1.4, and TR1.8, this antibody maps to the immunodominant region of TPO recognized by autoantibodies in patient sera (Nishikawa et al., 1994; PMID: 7512572). This antibody has also been employed in flow cytometry to detect cells expressing surface TPO (Jaume et al., 1999; PMID: 10323395).
Antibody First Published
Chazenbalk et al., 1993. Human organ-specific autoimmune disease. Molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic immunodominant region and restricted immunoglobulin gene usage in the target organ. J Clin Invest. 1993 Jul; 92(1): 62–74. PMID:7686925
Note on publication
Describes the original production of the antibody and its characterisation through competition assays.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
