Cat. No.
MABL-3077
Application
Blocking, FACS, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MT251
From
Recombinant Antibody
Specificity
The original antibody is specific for TIGIT. The antibody does not cross-react with LAG-3, PD-1 and PD-L1.
Alternative Names
VSTM3; VSIG9; WUCAM; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain- containing protein 3
UniProt
Q495A1
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant human TIGIT protein.
Application Notes
The original mouse antibody was subjected to humanized modification, keeping a similar binding activity. The specificity of the original and the humanized format of the antibody was confirmed by ELISA analysis (EC50= 0.09901 μg/mL and 0.07412 μg/mL respectively). The original format of antibody blocked the binding activity of TIGIT and CD155. The original format and the humanized version of the antibody could recognize the TIGIT antigen expressed by Jurkat/TIGIT cells by FACS analysis. The original format of the antibody was able to effectively block the binding of TIGIT to CD155 (CN112794909).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
