Cat. No.
MABL-3098
Application
neutralizing, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
chTL1A-2
From
Recombinant Antibody
Specificity
The antibody binds specifically to chicken TL1A, a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. The antibody does not react with the chIL-8/pET32a or proteinase K-pretreated chTL1A, verifying its specificity for the intact chTL1A.
Alternative Names
TNFSF15; TNF-like ligand 1A; tumor necrosis factor superfamily 15; chicken TL1A; Tumor necrosis factor-like ligand 1A; chTL1A; TL1A mAb-2
UniProt
Q50L61
Immunogen
The original antibody was raised by immunizing BALB/c mice with recombinant TL1A protein.
Application Notes
The specificity of this antibody (IgG1) was confirmed by ELISA analysis (Lee et al, 2014; pmid: 24565040). The antibody was used as a detecting antibody in capture ELISA assay to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens. The antibody detected the recombinant chicken TL1A by western blot analysis. Furthermore, the antibody inhibited chTL1A induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9) (Lee et al, 2014; pmid: 24565040).
Antibody First Published
Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken TL1A. Vet Immunol Immunopathol. 2014;159(1-2):103-9. PMID:24565040
Note on publication
The paper describes the generation and characterization of the antibody, specific for chicken TL1A.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
