Cat. No.
MABL-3124
Application
BLI, neutralizing, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Clostridium difficile
Clone No.
A20.1
From
Recombinant Antibody
Specificity
The antibody is specific for toxin A of Clostridium difficile. It recognises a conformational epitope on native C. difficile toxin.
Alternative Names
TcdA; EC:3.4.22
UniProt
P16154
Immunogen
The original antibody was generated by immunizing a llama with recombinant RBD fragments TcdA-RBD-f1 and TcdB-RBD-f1, followed by phage display library construction and panning against TcdA- RBD-f1.
Application Notes
The specificity of the original format of the antibody to TcdA was confirmed by ELISA analysis. The binding affinity of the original format of the antibody was measured by surface plasmon resonance (Kd= 2 nM). Human lung fibroblast (HLF) cytotoxicity assay was used to determine whether the original format of the antibody could neutralize TcdA-induced HLF cell rounding. The antibody showed neutralizing activity to TcdA (US9771416B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
