Anti-CD62L mAbs

Cat. No.

MABL-1057

Name

Anti-CD62L mAbs

Clone No.

AFD- LAM1-116

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, FC, IF, IHC

Species Reactivity

Guinea Pig, Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for the natural   ligand binding domain of L-selectin.

Alternative Name

SELL; L-selectin; LAM-1; LECAM1; Leukocyte   surface antigen Leu-8; Leukocyte adhesion molecule 1; Lymph node homing   receptor; CD62 antigen-like family member L; Leukocyte-endothelial cell   adhesion molecule 1

UniProt

P14151

Immunogen

This original antibody was raised by   immunizing a L-selectin deficient mouse with L-selectin.

Application Notes

The original antibody was used for flow   cytometry on Holstein calve and sheep leukocytes (Leid et al., 2001;   PMID:11290767), on EDTA-anticoagulated guinea pig blood (Fryer et al., 1997;   PMID:9109449), an d on plasma samples from rats (Yan et al., 2000;   PMID:10803628). This antibody was used for immunoprecipitation of L-selectin   from bovine lymphocyte lysates (Leid et al., 2001; PMID:11290767), and on RBL   cells (Haribabu et al., 1997; PMID:9153259). This antibody was used to test   the effect of the antibody on NF-kappa B activation. It was shown that this   antibody could indeed activate NF-kappa B in neutrophils (Turutin et al.,   2003; PMID:12586371). This antibody was used for immunohistochemistry on a   rat endometriosis model and on tissues of human endometriosis. This antibody   was able to show the localization of the protein (Odagiri et al., 2007;   PMID:17482182). This antibody was used for immunofluorescence on RBL cells   where it showed staining like expected (Haribabu et al., 1997; PMID:9153259).

Antibody First Published

Steeber et al. Ligation of L-selectin   through conserved regions within the lectin domain activates signal   transduction pathways and integrin function in human, mouse, and rat   leukocytes J Immunol. 1997 Jul 15;159(2):952-63. PMID:9218616

Note on publication

Since ligation of L-selectin through its ligand recognition region may mimic physiologic ligand binding, the original paper  generated a new panel of mAbs that engaged a conserved ligand-binding region   within the lectin domains of human, mouse, and rat L-selectin.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.